HIFU联合相变氟碳乳剂损伤兔肝脏的二维超声实时监控

Real-time monitoring with two-dimensional ultrasonography in rabbit liver damaging experiment using HIFU combining with a phase shift perfluoropentane emulsion

目的探讨二维超声实时监控HIFU(high intensity focused ultrasound),联合相变氟碳乳剂损伤兔肝脏的可行性。方法使用同一聚焦超声换能器,辐照深度、功率一致条件下,静脉注射等量相变氟碳乳剂(实验组)或生理盐水(对照组)后HIFU辐照兔肝脏,测量辐照靶区的灰度值、回声增强区的大小,解剖测量凝固性坏死灶的大小。结果对照组HIFU辐照兔肝脏所产生的灰度值、回声增强区体积和最大面积小于实验组,凝固性坏死灶的最大截面积、体积也小于实验组,能效因子大于实验组。回声增强区最大面积与凝固性坏死灶最大截面积、体积之间存在定量关系。结论二维超声可实时监控相变氟碳乳剂存在条件下的HIFU损伤,相变氟碳乳剂提高了HIFU的损伤效率。

Objective To explore the feasibility of real-time monitoring in the two-dimensional ultrasonography in damaging experiment using high intensity focused ultrasound （HIFU） and combining with phase shift perfluoropentane emulsion. Methods Using a identical transducer, under same radiating depth and emission power, isovolumetric phase shift perfluoropentane emulsion （as experimental group） and physiological saline （as control group） were injected intravenously prior to rabbit liver ra- diating with HIFU, respectively. Grey scale of target region, maximal secar and three dimension length of hyperechoic region were measured using software of JC MODE HIFU TUMOR TREATMENT SYSTEM. Length, width and thickness of necrotic region were measured posterior to dissection the liver three days later. Results Grey scale of target region, volume and maximal secar of hyperechoic region, volume and maximal secar of necrotic region in control group were less than that in experimental group. Energy efficiency factor （EEF） in control group was more than that in experimental group. There were quantification relationship existing between maximal secar of hyperechoic region and maximal secar and volume of necrotic region. Conclusion Two-dimensional ultrasonography could monitor damage of HIFU combined with phase shift perfluoropentane emulsion in time, the damaging efficiency of HIFU was elevated by phase shift perfluoropentane emulsion.