摘要
探讨了Bacillus fusiformis作为处理制革中Cr（Ⅵ）的一种新生物材料的可行性。首先在LB培养基中加入Cr（Ⅵ），检测了Bacillus fusiformis在含Cr（Ⅵ）培养基中生长后细胞内外的Cr（Ⅵ）和总铬含量；然后研究了不同Cr（Ⅵ）初始浓度、温度、pH值、细胞生物量对Bacillus知iformis处理Cr（Ⅵ）的影响，并在最适温度、pH值、细胞生物量条件下，对菌株连续培养以检验其对Cr（Ⅵ）的持续还原能力；最后探究了制革中常见金属离子对Cr（Ⅵ）还原的影响。结果表明：Bacillus fusiformis处理Cr（Ⅵ）的方式可能主要为胞外还原。当初始Cr（Ⅵ）浓度不超过50mg／L、温度32—37℃、pH值7．0～8．0、细胞生物量6．7×10^11 CFU／mL时，Cr（Ⅵ）的去除率较大。在连续培养过程中，菌株具有持续的生长和还原Cr（Ⅵ）的能力。在各种金属离子的比较中得出Cu^2＋对Bacillus fusiformis处理Cr（Ⅵ）有较强的促进作用，Al^3＋、Fe^3＋次之，Cr^3＋有一定的抑制作用。研究表明，Bacillus fusiformis的还原特性可为处理制革中的Cr（Ⅵ）作参考。
Bacillus fusiformis was detected for its ability to remove Cr（ Ⅵ ） in leather manufacture. Cr（ Ⅵ ） was tested inside and outside of the Bacillus fusiformis after its growth in Luria broth. Then, the Bacillus fusiformis was used to remove the Cr（Ⅵ） in Luria broth at different initial Cr（Ⅵ） concentration, temperature, pH and biomass to get better conditions. At the optimum condition, Bacillus fusiformis in Luria broth was continuous cultured to detect the reduction capacity of Cr （ Ⅵ ）. Finally, the effect of main metal ions in leather manufacture on Cr（ Ⅵ ） was researched. The results showed that Cr （ Ⅵ ） reductase generated by the Bacillusfusiformis was mainly extracellular. The removal rate of Cr（ Ⅵ ） was the higher at the condition of temperature 32 - 37 ℃, pH = 7.0 - 8. 0, and biomass 6.7×10^11 CFU/mL. In the process of continuous culture, the strain showed continuous growth and removal ability of Cr（ Ⅵ ）. In respect of the effect of the metal ions on Bacillus fusiformis, Cu^2＋ , Al^3＋ and Fe^3＋ actived the reduction capacity of Bacillusfusiformis to Cr（ Ⅵ ）, while Cr^3＋ inhibited the reduction capacity to Cr（ Ⅵ ）. The experiments demonstrated that the Bacillus fusiformis could be selected as a new biomaterial to remove Cr（ Ⅵ ） in leather manufacture.
出处
《皮革科学与工程》
CAS
北大核心
2009年第5期18-22,共5页
Leather Science and Engineering
