青梗菜优异DH系的创制与利用

Obtaining and Utilization of DH Lines in Pakchoi (Brassica rapa L.ssp.chinensis L.)

【目的】创制多样性的青梗菜DH系,应用于杂种优势育种实践。【方法】以15个优良青梗菜杂交种为试材进行游离小孢子培养,通过优化培养条件建立高效培养体系。【结果】获得了373株小孢子植株;鉴定出251株双单倍体;筛选出7个优异DH系;配制出2个优良组合。【结论】建立了利用游离小孢子培养技术创制青梗菜DH系及其杂种优势育种的技术体系。

Objective The study is to develop diversified DH lines in pakchoi for the practice of heterosis breeding.Method Isolated microspore culture was employed in 15 hybrid varieties.An efficient culture system was established through the optimization of culture conditions.Result Totally 373 regenerated plants were obtained,in which 251 were identified as double haploid.Furthermore,seven excellent DH lines were screened out.Two improved F1 combinations were made based on such DH lines.Conclusion A technical system was successfully established for heterosis breeding of pakchoi with DH lines as parents created by isolated microspore culture.