TGF-β受体mRNA在湖羊机体组织中的表达水平及其与排卵数的关系

Relationship Between the mRNA Expression Level of TGF-β Receptors in Hu Sheep's Tissues and the Number of Ovulation

【目的】通过分析TGF-β受体(TGF-βRⅠ、TGF-βRⅡ)mRNA组织表达谱及其表达水平与湖羊排卵数的关系,研究TGF-β受体对湖羊排卵数的影响。【方法】挑选16头经产母羊,其中8只产多羔的为高产组,8只产单羔作为对照组,使用氯前列醇钠法进行同期发情,发情后48～60h内屠宰,测定排卵数;分离取高产组母羊的下丘脑、垂体等组织样进行组织表达谱分析,用实时荧光定量PCR法检测湖羊卵巢TGF-βRⅠ和TGF-βRⅡ基因mRNA表达水平,分析TGF-β受体表达水平与湖羊排卵数的关系。【结果】TGF-βRⅠ在生殖器官相对表达量极显著高于肺和肌肉(P<0.01),TGF-βRⅡ在生殖器官相对表达量极显著高于其它组织(P<0.01),是卵巢高表达基因;高产组湖羊卵巢组织TGF-βRⅠ与TGF-βRⅡ基因mRNA表达量分别极显著(P<0.01)与显著(P=0.011)高于对照组;湖羊卵巢组织TGF-βRⅠ和TGF-βRⅡ基因mRNA表达量与排卵数呈正相关,相关系数分别为0.562(P>0.05)和0.711(P<0.05)。【结论】TGF-β受体在卵巢组织中表达高,其中高产组TGF-βRⅠ和TGF-βRⅡ基因mRNA表达量极显著或显著高于对照组,而且与排卵数呈正相关。

Objective The impact of TGF-β receptors（TGF-βRⅠ,TGF-βRⅡ）on the number of ovulation was studied by analyzing the tissue expression pattern and the relationship between the mRNA expression level of TGF-β receptors and the number of ovulation.Method Sixteen multiparous ewes were selected to investigate,eight which bred multi-lamb were used as the high-yield group,the other eight which bred single lamb were used as control group.Cloprostenol sodium were injected to make the synchronization of estrus treatment,then slaughtered within 48-60 h after empathema, two side ovaries were collected after slaughtering, the number of corpus rubrum on the ovaries was counted to deternine the number of ovulation; the tissues such as hypothalarnus, hypophysis were collected from one ewe in the high-yielding group, to make a tissue expression analysis. The relationship between the mRNA expression level of TGF-β receptors and the number of ovulation, which were detected by real-time fluorescent quantitative PCR, were analyzed. [Result] TGF-βR I＇s relative expression level in the reproductive organ was only significantly higher than in the lung and muscle （P〈0.01）. TGF-βRH＇s relative expression level in reproductive organ was significantly higher than that of other tissues （P〈0.01）, it is a high-expressed gene in ovary. TGF-βR I and TGF-βRll＇s mRNA expression level in the ovaries of the high-yield group were significantly higher （P〈 0.01） and obviously higher （P=0.011） than the control group, respectively. TGF-βR I and TGF-βR II＇s mRNA expression level have a positive correlation with the number of ovulation, the correlation coefficient were 0.562 （P〉0.05） and 0.711 （P〈0.05）, respectively. [Conclusion] TGF-β receptors showed the high level in ovary, TGF-βR I and TGF-βRII＇s mRNA expression level in the ovaries of the high-yielding group were extremely or obviously higher than the control group, furthermore, it had a positive correlation or a significant positive correlation with the number of ovulation, so it illusted that TGF-β receptors have a close relationship with high-yielding Hu sheep.