摘要
【目的】研究致瘤性病毒REV、ALV-J单一感染和共感染肉鸡后血液和脾T淋巴细胞的免疫功能与脾组织病理学的变化。【方法】用一定剂量的REV和ALV-J单一感染及共感染1日龄肉鸡,取感染后不同日龄鸡的血液和脾组织,无菌分离淋巴细胞,用3H-TdR掺入法和MTT法分别检测T淋巴细胞增殖活性和细胞毒T淋巴细胞(CTL)的杀伤活性,并对脾脏进行组织切片、H.E染色检测组织病理学变化,通过免疫荧光抗体结合试验(immunofluorescence assay,IFA)分析组织病毒感染量变化。【结果】感染REV和ALV-J的肉鸡血液和脾T淋巴细胞的增值活性和CTL杀伤活性在整个感染监测期出现降低,单一感染比,共感染两种病毒的肉鸡在某些阶段出现T淋巴细胞免疫功能抑制加重;检测感染后17d和37d的脾脏组织病理学变化表明感染病毒的脾脏组织出现间质稀疏,淋巴细胞数量减少,生发层被破坏或减少,17d较37d出现病理变化更为明显;同时采用荧光标记的单克隆抗体进行IFA检测发现脾内淋巴细胞含有大量病毒粒子,在共感染两种病毒的肉鸡脾细胞内均检测出两种病毒,且含有病毒数量明显多于单一感染一种病毒的肉鸡。【结论】REV和ALV-J共感染后肉鸡T淋巴细胞功能抑制更为严重,这可能与两种病毒在肉鸡体内数量积聚增加、互为促进有重要关系;同时这两种病毒感染后造成T免疫细胞增殖活性和对肿瘤细胞的杀伤活性降低,可能是病毒持续感染、增殖及组织病变、产生肿瘤细胞的前提,本研究为家禽临床生产中防治REV和ALV-J感染提供免疫学基础。
Objective To study the effect of single-and co-infection with REV and ALV-J on T lymphocytes bioactivities and histopathology in broiler chickens.Method Blood and spleen T lymphocytes bioactivities including lymphoproliferation responses and cytotoxicitic responses and pathological histopathology of spleen were detected in broiler chickens singl-or co-infected with REV and ALV-J at different days post inoculation,the viruses expression in infected broiler s spleen were detected with immunofluorescence assay (IFA). [Result] The results indicate that, blood and spleen T lymphocytes proliferate responses and cytotoxicity in broilers infected with REV or/and ALV-J were inhibited in the whole observed period compared with controls, in the co-infected chickens they were inhibited more severely than in the single-infected. The histopathology of spleen in infected chickens at 17 and 37 days post inoculation indicated that cell interium increased, the numbers of lymphocytes decreased and the regrowth were destroyed or decreased, especially more significiant at 17dpi than at 37 dpi. The different numbers of virus were detected in spleen lymphocytes in REV-infected and/or ALV-J-infected chickens, respectively. In co-infected chickens' spleen, both REV and ALV-J were detected and the total numbers of viruses were more than in chickens single-infected with REV or ALV-J. [ Conclusion ] It can be concluded that co-effect of REV and ALV-J can cause more immunosuppression on T lymphocytes bioactivities in broiler chickens than single-effect of ALV-J or REV, which contribute to the sever histopathology and the product of tumor cells. This will be helpful for understanding of the effect of co- or dulpti-infection with many viruses and control them in poultry.
出处
《中国农业科学》
CAS
CSCD
北大核心
2009年第9期3296-3304,共9页
Scientia Agricultura Sinica
基金
农业公益性行业科技研究专项(200803019)
山东农业大学青年科技创新基金(23477)