加味四逆散抗肝纤维化大鼠机理研究

Study of mechanism on counteraction of Jiawei Sini San on hepatic fibrosis in rats

目的观察加味四逆散对肝纤维化大鼠的预防效果并探索其防治机理。方法采用四氯化碳(CCL4)植物油溶液和酒精复制大鼠肝纤维化模型,观察加味四逆散对肝纤维化大鼠肝窦内皮细胞(SEC)窗孔大小及层黏蛋白(LN)表达的影响。结果加味四逆散组大鼠SEC壁LN的表达明显减弱,SEC失窗孔减轻,窗孔变大,与模型组比较差异有高度统计意义(P<0.01)。结论加味四逆散抗肝纤维化的机制之一是通过阻止SEC膜上筛状窗孔变小,降低LN表达,进而抑制肝窦毛细血管化来实现的。

Objective To observe the preventive effect of Jiawei Sini San （JWSNS） on hepatic fibrosis in rats, and explore the mechanism of prevention and treatment. Methods The models of hepatic fibrosis in rats were established by CCL4 vegetable oil and alcohol, and observed the effect of JWSNS on SEC fenestra size and LN of rats with hepatic fibrosis. Results The LN expression of SEC wall in the rats of JWSNS group obviously weakened, and SEC fenestra was bigger. Compared with model group, there was a very significant differenee（P 〈 0.01 ）. Conclusion JWSNS can treat hepatic fibrosis by stopping the cribriform fenestra of SEC membrane changing smaller,and reducing LN expression, and then inhibiting the sinusoidal capillaries to achieve.