摘要
以黄瓜(Cucumis sativus L.)哈研3号为试验材料,采用组织培养技术系统探讨以子叶或子叶节为外植体时不定芽的诱导效率,最终确定以子叶节作为再生体系及遗传转化体系的试验材料;并系统探讨了不同PGRs浓度对不定芽诱导、不定芽伸长及生根阶段的影响;建立了哈研3号黄瓜的高频再生体系;并在子叶节根端膨大阶段进行了抗生素(Km)浓度的摸索,建立了较为适宜的遗传转化体系,为转基因工作打下坚实的基础。
Taking cucumber （Cucumis sativus L.） ＂Hayan No. 3＂ as the test material,using tissue culture technology system to explore induction efficiency of adventitious shoot for cotyledon or cotyledon node as explants. Then.the cotyledon node as a regeneration syslem and genetic transformation system was determined as test materials. ,And systems of different PGRs concentration on adventitious bud induction.adventitious shoot elongation and rooting stages of impact were discussed. Final ly.tbe＂Hayan No. 3＂ the high frequency regeneration system of cucumber was set up. Cotyledon node at lhe root end-stage expansion of the antibiotic （Kin） concentration was explored,and a more appropriate genetic transformation system was constructed for genetically modified job thus laying a solid foundation.
出处
《黑龙江农业科学》
2009年第5期5-7,共3页
Heilongjiang Agricultural Sciences
关键词
黄瓜
子叶节
不定芽
再生体系
遗传转化体系
PGRs
cucumber
cotyledon node
adventitious shoot
regeneration system
genetic transformation system
PGRs
