嗜水气单胞菌微量凝集反应条件的研究

Study on the Reaction Condition in Micro-agglutination Test of Aeromonas hydrophila

应用灭活嗜水气单胞菌作为凝集原，兔源抗血清作为凝集素，比较了在不同条件下微量凝集反应的结果。当菌液浓度约为１０８／ｍｌ，每孔反应液为３０μｌ时，与常规的玻板凝集反应比较，可提高２～３个血清滴度。应用振荡器可加快反应，在１０ｍｉｎ内就可判读结果。温度（在１０～３７℃范围内）的影响不明显。应用该法和玻板凝集法同步检测免疫鱼鳖血清的结果表明，该法不仅具有敏感性高、提高检测率的优点（比平板法提高３２％），而且还能定量检测鱼鳖血清抗体滴度和快速进行细菌鉴定（筛选鉴定了４株嗜水气单胞菌和６株水气单胞菌），为流行病学调查、免疫效果观察和细菌鉴定提供了敏感和可靠方法。

A micro-agglutination test was established with inactivated Aeromonas hydrophila (Ah) and rabbit anti-Ah-serum, and that the optimum reaction condition was investigated This method to measure serum antibody was more sensitive than slide agglutination test under the reaction condition with a bacterial density of 10 8/ml, and a total volume of antigen and antibody of reaction liquid of 30 μl/well, temperature range of 25～37℃ and reaction-time of 30 minutes, namely it could improve 2～3 titre and 32% of detection rate in comparison with the slide agglutionation In addition, as a quantitative measure method, it could be used to investigate epidemilogy of infection and to determine the immune effect after vaccination.