桑蚕组织液中酸性磷酸酶、过氧化物酶同工酶研究

Perliminary Report on Acid Phosphatase (APS) and Peroxidase (PER) Isozyme in Silkworm Tissue

采用超薄层聚丙烯酰胺等电聚焦电泳法分析，各品种家蚕幼虫组织液中酸性磷酸酶同工酶（ＡＰＳ）在ｐＨ４．７～５．４之间均有一系列相似性的强带；不同发育时期ＡＰＳ同工酶差异显著。过氧化物酶同工酶（ＰＥＲ）主带分布于ｐＨ４．１～４．２和ｐＨ７．７两处，且ｐＨ４．１～４．２之间的同工酶强度显著大于ｐＨ７．７处；不同发育时期的差异主要表现在ｐＨ７．７部分。

The Acid phosphatase and Peroxidase isozymes in silkworm larval tissue were investigated by means of isoelectric-focus polyacrylamide gel electrophoresis and scanner.The results showed that all the APS isozyme patterns of used races had similar major belts among pH 4 7～5 4 and there were differences with larval development.But for the peroxidase isozyme all major belts appeared in pH 4 1～4 2 and pH 7 7,respectively,also the major belts in pH 4 1～4 2 were stronger than those in pH 7 7 and differences among races could be observed mainly in pH 7 7.