固态发酵柠檬酸渣转化为多酶蛋白饲料的研究

Studies on the Production of Multi-Enzyme Protein Feed From Citric Acid Waste With Solid StateFermentation

研究了用黑曲霉Ａ１、Ａ１５和酵母Ｙ７固态发酵柠檬酸渣生产多酶蛋白饲料。在３０℃的最适温度下，３株菌种混合进行固体发酵，粗蛋白增加量（绝干）约为１６％～１８％，酸性蛋白酶活性５１３９ｕ／ｇ，纤维素酶活性达６１ｕ／ｇ。试验结果表明：该酸性蛋白酶在１００℃加热１ｈ有８０％的酶存活，系耐高温酸性蛋白酶。

Production of multi-enzyme protein feed was studied on citric acid waste with Aspergilhus niger A 1、A 15 and Yeast Y 7 by solid state fermentation.At the optimal temperature 30℃,the total protein content increased about 16%～18%,acid proteinase activity was 5139 u/g.cellulase activity was 61 u/g。80% of the acid proteinase activity was still living at the temperature of 100℃,heated for l h.It is a heat-resisting acid proteinase.