2型糖尿病患者外周血循环祖细胞的变化和临床意义

The Changes and Clinical Significance of Circulating Progenitor Cells in Patients with Type 2 Diabetes

目的探讨2型糖尿病(T2DM)患者外周血中循环祖细胞(CPCs,以CD34+细胞为分子标志物)的变化,并分析其与空腹血糖(FPG)、糖化血红蛋白(HbA1c)、超敏C反应蛋白(hsCRP)之间的相关性。方法采用流式细胞仪检测22例健康体检者(A组)、38例无并发症T2DM患者(B组)、32例T2DM合并微血管病变患者(C组)外周血CD34+细胞数量,同时检测3组外周血FPG、HbA1c和hsCRP水平。结果B、C组患者外周血CD34+细胞数量均降低,与A组相比,其CD34+、FPG、HbA1c、hsCRP水平差异均有统计学意义(均P<0.05)。外周血CD34+细胞数量与FPG、HbA1c、hsCRP均呈负相关。结论CPCs在一定程度上参与了T2DM及其微血管病变的发生、发展,可作为T2DM病情变化及其微血管病变的监测指标。

Objective To explore the changes of circulating progenitor cells （CPCs,characteristic of CD34^＋） in peripheral blood and analyze the correlation between CPCs and fasting plasma glucose （FPG）, glycosylated hemoglobin （HbA1c）, high sensitive C reactive protein （hsCRP） in type 2 diabetic patients （T2DM）. Methods We detected peripheral blood CD34^＋ cells by flow cytometry in 22 healthy volunteers （group A）,38 T2DM without complications （group B） and 32 T2DM with micro-vascular disease （group C）. Moreover, we also measured other parameters of peripheral blood,such as FPG, HbAlc,hsCRP. Results The CD34^＋ from group B and group C were reduced. Compared with group A, there were significant differences in CD34^＋ , FPG, HbAlc, hsCRP in group B and group C（all P〈0.05）. An inverse correlation was found between CD34^＋ and FPG,HbA1C,hsCRP. Conclusion The reduction of CPCs was associated with development of micro-vascular complication in T2DM. The number of CPCs is a predictor in the progress of T2DM and its micro-vascular complications.