摘要
本文研究了模板DNA浓度、镁离子浓度、引物浓度、dNTP及酶含量、退火温度及反应缓冲液中明胶或BSA含量等对ISSR反应体系的影响,通过优化反应条件初步建立了适合于剑麻ISSR反应的扩增体系,为进一步利用ISSR分子标记开展剑麻遗传多样性分析,种质资源鉴定和分子标记辅助育种等研究提供参考。
The main influencing factors of ISSR were studied and the experiment parameters were optimized. By adjusting the concentrations of DNA template, Mg^2＋, dNTP,primer, the contents of Taq DNA polymerase, annealing temperature and PCR buffer （BSA or gelatin plus/free）, a satisfactory ISSR reaction system for sisal was established. The results would provide reference for genetic diversity analysis, germplasm identification and marker-assisted selection of sisal with ISSR marker.
出处
《中国麻业科学》
2009年第5期291-295,共5页
Plant Fiber Sciences in China
基金
中国热带农业科学院南亚热带作物研究所中央级公益性科研院所基本科研业务费专项(sscri200802)
国家麻类产业技术体系建设专项资金资助项目(项目编号:nycytx-19-E02)
2007年公益性行业(农业)科研专项(nyhyzx07-018)
关键词
剑麻
ISSR
sisal
inter-simple sequence repeat （ISSR）
