经皮冠脉内微栓塞对比格犬心功能的影响

Effect of percutaneous coronary microembolism on dog heart function

目的通过观察经皮冠脉内微栓塞对比格犬心功能的影响,探索建立慢性心力衰竭(轻-中度)犬模型的技术。方法实验动物为32条比格犬,对犬冠状动脉中远端反复微栓塞,每周1次神经内分泌因子测定、血液动力学和超声心动图检查,直到左心室整体射血分数(EF)、左室内压力上升最大速率分别减少20%～25%,左室舒张末压>14mmHg(1mmHg=0.133kPa),且上述指标稳定2周以上。结果19条比格犬达到上述标准;Masson三色染色结果提示动物左室梗死区域可见灶性心肌细胞坏死和间质纤维化。结论经皮冠状动脉反复微栓塞可成功建立轻-中度慢性心力衰竭犬模型。

Objective To establish a chronic heart failure （CHF） model of dogs by observing the effect of percutaneous coronary microembolism on dog heart function. Methods Thirty-two dogs were included in this study. Baseline assessment of their heart function was performed in a conscious state. Microsphere was injected into the coronary via a coronary catheter. CHF[20%- 25% reduction in EF, dP/dtmax and Left ventricular end-diastolic pressure （LVEDP）〉14mmHg]was induced by weekly coronary microembolization. Results CHF was successfully induced in 19 dogs. Conclusion CHF can be induced by weekly coronary microembolization via a coronary catheter, which is similar to the human CHF in manifestations and pathogenesis.