两种低温保护剂对皮肤储存后α-辅肌动蛋白表达及活力的影响

Effect of two cryoprotectants on expression and viability of α-actinin in human skin

目的比较两种不同低温保护剂对皮肤组织α-辅肌动蛋白(α-actinin)低温保存后表达的影响,为寻求皮肤组织低温保护剂的最佳配方提供实验依据。方法新鲜成人皮肤组织分为3组,新鲜对照组和海藻糖-二甲基亚砜(T-D)组、二甲基亚砜-丙二醇(D-P)低温保护剂保存组,-196℃液氮冻存7d、14d复温,免疫组织化学染色对各组间皮肤进行比较。同时对各组皮片进行氧耗量测定。结果0.5mol/L海藻糖-二甲基亚砜能够很好保护皮肤组织,α-actinin的表达量与新鲜皮肤组相似。结论海藻糖与二甲基亚砜联合应用对皮肤组织α-actinin的保护作用优于传统组。

Objective To compare the effects of different cryoprotectants on expression of α-actinin in human skin. Methods Fresh skin tissues were divided into control group, trehalose group, and DMSO group. DMSO and propylene glycol used as cryoprotectants were stored in liquid nitrogen at - 196℃ for 7 days and re-warmed after 14 days. The structure of skins in different groups was compared with immunohistochemical staining. Oxygen consumption of skin grafts was measured. Results Trehalose and DMSO could protect skin tissues. The expression level of α-actinin in cryopreserved skin tissues was similar to that in fresh skin tissues. Conclusion The protective effect of trehalose in combination with DMSO on skin tissues is better than traditional drugs.