苦瓜蛋白对柯萨奇3型病毒感染大鼠心肌细胞的保护作用

Protective Effect of Momordicin on Coxsackievirus B3 Infected Rat Cardiocyte

目的在病毒性心肌炎心肌细胞模型上观察苦瓜蛋白对柯萨奇3型病毒(CVB3)感染SD大鼠心肌细胞的保护作用及其机制。方法体外原代培养SD大鼠心肌细胞,以CVB3感染培养大鼠心肌细胞,复制病毒性心肌炎心肌细胞模型,分别测定心肌细胞搏动百分比(PBC)、半数有效率(ED)、细胞病变(CPE)、心肌酶谱、半感染致死量(TC ID50),分析其对心肌细胞的保护作用。结果苦瓜蛋白的ED值为0.045 mg/mL,80、40、20、10μg/mL苦瓜蛋白可显著提高心肌细胞PBC、降低病毒的复制,80、40μg/mL苦瓜蛋白可减少CPE,降低心肌酶水平。结论苦瓜蛋白对感染CVB3的心肌细胞有较强的保护作用。

Objective To observe the protective effect of momordicin on Coxsackievirus B3 infected rat cardiocyte and its mechanism. Methods Rat cardiocyte were prime cultured in vitro, then infected with Coxsackievirus B3, and the viral myocarditis model was duplicated, the protective effect of momordiein was analyzed by measuring median effective dose （ED50） , percents of beat cells（ PBC） , cytopathic effect （CPE） , myocardiocyte related enzymes and tissue culture infective dose（TCIDS0）. Results A distinct protective effect of momordicin on CVB3 bffeeted rnyocardioeyte was found（ED50 = 0. 045 mg/mL）. At the concentration of 80,40,20,10 μg/mL, distinct effects of enhancing PBC, reduction of CPE （ cytopathie effect） and replication of CVB3, and at the concentration of 80,40 μg/mL, LDH and AST were reduced remarkably. Conclusion Momordicin has a strong protective effect on CVB3 infected cardiocyte.