罗格列酮诱导裸小鼠胃癌移植瘤细胞分化

Induction Differentiation of Rosiglitazone in Human Gastric Carcinoma Transplanted into Nude Mice

目的研究罗格列酮(ROS)对人胃低分化黏液癌MGC-803细胞裸鼠移植瘤的诱导分化作用,初步探讨其可能机制。方法培养人胃癌MGC-803细胞,建立移植瘤模型,随机分为模型组、维甲酸组、ROS 25mg/kg组、ROS 50 mg/kg组和ROS 100 mg/kg组,用药后,观察移植瘤体积变化并计算抑瘤率;检测肿瘤细胞周期及胃粘蛋白(Mucin 5AC)。结果ROS能缩小移植瘤体积,肿瘤细胞被阻滞于G0/G1期,S期细胞减少;Mucin5AC表达增强。结论ROS诱导胃癌裸小鼠移植瘤细胞分化,可能是通过抑制胃癌细胞从G1期向S期过渡,降低细胞的增殖能力实现的。

Objective To investigate the induction differentiation effects of human gastric carcinoma which human gastric lower - differentiation mucinous carcinoma MGC - 803 cells transplanted into back subcutaneous of nude mice by using rosiglitazone （ ROS ）, and to preliminarily explore the mechanism of differentiation of ROS. Methods Firstly, cultivated the MGC - 803 cells and constructed the model of nude mice gastric transplanted cancer. Secondly, The mice were divided into five groups ： model, ATRA 11 mg/kg, rosiglitazone 25 mg/kg, rosiglitazone 50 mg/kg, rosiglitazone 100 mg/kg. After that the tumor volumes were measured and inhibition tumor rates were calculated ;the cell cycle was detected by FCM; the protein expression level of Mucin SAC was detected by Results The volume of tumor decreased significantly in ROS group. Xenograft tumor cells were arrested in G0/G1 stage, and the cells in S stage decreased significantly, and up - regulated Mucin 5AC protein expression. Conclusion The maganism of ROS induced gastric carcinoma MGC - 803 cells which transplanted in nude mice differentiation was possibly that ROS inbited carcinoma cells from G1 stage to S stage and depressed their proliferation.