Hela培养上清液对hUVEC细胞增殖的影响

The Effect of Supernatant from Cultured Hela Cells on Cell Proliferation of hUVEC

目的研究子宫颈癌细胞(Hela)的培养上清液对人脐静脉内皮细胞(hUVEC)增殖的影响。方法以Hela细胞培养上清液和DMEM培养基的等比例混合液培养的hUVEC细胞为实验组,以纯DMEM培养基培养的hUVEC细胞为对照组。MTT法检测细胞增殖情况的变化;流式细胞术检测细胞周期的改变;PCR法检测CDK和CyclinD调控基因的表达情况。结果hUVEC经Hela细胞培养上清液作用96 h后,细胞数明显增加,S期细胞的比例明显上升,CDK和CyclinD调控基因表达明显上调。结论Hela细胞培养上清液能明显促进人脐静脉内皮细胞的增殖,其机制之一可能是通过上调CDK和CyclinD调控基因来诱导更多的细胞进入S期,从而导致细胞的快速增殖。

Objective To research the effect of supernatant from cultured Hela cells on cell proliferation of hUVEC. Methods Experiment group was treated with the mixed liquor of Hela cells supernatant and DMEM medium in geometric proportion, control group was treated with DMEM medium. The change of cell proliferation was detected by MTr method, the change of cell cycle was detected by flow cytometry, and CDK and CyclinD gene were detected by PCR method. Results After incubation with the supernatant from cultured Hela cells, the population of hUVEC cells increased obviously, more hUVEC cells enter into S stage of cell cycle, the expression of CDK and CyelinD genes had clearly up - regulation. Conclusion The supematant from cultured Hela cells could effectively enhance the proliferation of hUVEC cells. One of the mechanisms may be to induce more cell enter into S stage of cell cycle by up - regulating the expression of CDK and CyclinD genes