抗病毒转基因木瓜的分子检测

Molecular detection of virus resistant transgenic papaya varieties

本研究测定了抗病毒转基因木瓜品系55-1、GMYK和华农1号的结构特征序列,并根据测序结果设计、合成了结构特异性检测引物,建立了55-1、GMYK和华农1号的结构特异性检测方法。为提高检测效率,将结构特异性检测引物和内源基因检测引物置于同一反应体系之中,建立了转基因木瓜品系的两重PCR检测方法。本研究建立的PCR检测体系可用于进口木瓜的检测,从中发现未经我国批准的转基因木瓜品系,为转基因产品的标识管理提供技术支持。

In the present study, we determined junction DNA sequences between Cauliflower mosaic virus （CaMV） 35S promoter and coat protein （CP） gene of transgenic papaya lines 55 - 1 and GM YK. Meanwhile, we sequenced the transition between CaMV 35S promoter and replicase （RP） gene of transgenic variety HuaNong - 1 commercialized in China. On the basis of determined junction sequences, construct specific methods were developed for detection of 55 - 1, GM YK and HuaNong - 1. Duplex PCR systems were also developed by combining endogenous and exogenous primers in one tube. Application of PCR systems established here allow more precise detection of transgenic varieties in imported papayas which are so far not approved in China.