高效液相色谱法测定金英胆舒颗粒中大黄素和大黄酚的含量

Determination of Emodin and Chrysophanol in Jinyingdanshu Granules by HPLC

目的:建立金英胆舒颗粒中大黄素和大黄酚的含量测定方法。方法:采用Shi m Pack C18柱,甲醇:0.1%磷酸溶液(80∶20)为流动相,检测波长为254nm。结果:大黄素的回收率为99.63%,RSD=0.79%(n=6);大黄酚的回收率为98.10%,RSD=2.04%(n=6)。结论:本法简便、快速、重现性好,可作为该制剂的定量分析方法。

Objectives:To establish a method for the determination of emodin and chrysophanol in Jinyingdanshu Granules by HPLC. Method:The column was Shim Pack C18 and the mobile phase was melhanol 0.1% phosphate acid (80:20),with the detecting wavelength at 254 nm. Result:The recovery rate of Emodin was 99.63%,RSD=0.79% while the recovery rate of Chrysophanol was 98.10%,RSD=2.04%. Conclusion:The method is simple and quick with good reappaerance,and can be used for the quantitative analysis of Emodin and Chrysophanol.