二十二碳六烯酸对人胰腺癌细胞增殖的抑制

Inhibitory effects of docosahexaenoic acid on the growth of incubated human pancreatic cancer cell lines

目的探讨二十二碳六烯酸（DHA）对人胰腺癌细胞株Patu8988和SW1990生长的作用。方法采用MTT法检测DHA作用后Patu8988和SW1990细胞的增殖，流式细胞术检测DHA作用后Patu8988和SWl990细胞的凋亡、细胞周期和肿瘤相关蛋白环氧合酶2（COX-2）的表达量。结果DHA作用人胰腺癌细胞株24、48、72h后，细胞的增殖受到明显抑制（P〈0．01），同时DHA能诱导细胞凋亡，随着作用时间延长和作用剂量增加，效果越明显。50μg／ml DHA作用24h后，胰腺癌细胞的COX-2表达量下降（P〈0．05）。结论DHA能有效地抑制胰腺癌细胞增殖，同时诱导细胞凋亡，可能与COX-2在胰腺癌细胞中的表达下调有关。

Objective To investigate the effects of docosahexaenoic acid （DHA） on the growth of human pancreatic cancer cell lines. Methods Human pancreatic cancer cell lines Patu8988 and SW1990 were treated with DHA. The cell proliferation was evaluated by MTr assay. Cell cycle, apoptosis, and cyclooxygenase-2 expression were evaluated by flow cytometry. Results After incubation of pancreatic cancer cell with DHA for 24 to 72 hours, cell proliferation significantly decreased （ P 〈 0. 01 ） and apoptosis increased, both of which were timedependent and dose-dependent. After incubation with 50 μg/ml DHA for 24 hours, cyclooxygenase-2 expression of pancreatic cancer cell lines significantly decreased （ P 〈 0. 05 ）. Conchisions DHA can inhibit the growth of human pancreatic cancer ceils via decreasing cell proliferation and inducing apoptosis. These effects may be associated with the decrease of cyclooxygenase-2 expression.