血管内皮生长因子+936C/T基因多态性与非霍奇金淋巴瘤的关联研究

Study of relation between vascular endothelial growth factor gene +936C/T polymorphism and non-Hodgkin lymphoma

目的探讨血管内皮生长因子(VEGF)+936C/T基因多态性与非霍奇金淋巴瘤(NHL)临床特征的关系。方法采用聚合酶链反应—限制性片段长度多态性(PCR-RFLP)方法检测NHL组431例及对照组400例VEGF+936C/T的基因型;将NHL患者又分为T细胞型组和B细胞型组,Ⅰ、Ⅱ期组和Ⅲ、Ⅳ期组,骨髓浸润组和无骨髓浸润组,进行基因型分布分析。结果NHL组与对照组VEGF+936C/T基因型分布差异无统计学意义(P>0.05);NHL患者T细胞型组TT基因型低于B细胞型组(2.0%vs 7.1%,P<0.05),携带VEGF+936C/T基因型可能增加B细胞淋巴瘤的发病;NHL患者Ⅲ、Ⅳ期组TT基因型低于Ⅰ、Ⅱ期组(3.4%vs 8.3%,P<0.05),NHL患者Ⅲ、Ⅳ期组T等位基因频率分布低于Ⅰ、Ⅱ期组(17.1%vs 22.7%,P<0.05),携带+936TT基因型及T等位基因与NHL患者低的临床分期相关;骨髓浸润TT基因型低于无骨髓浸润(2.2%vs 6.7%,P<0.05),携带+936TT基因型与减少骨髓浸润相关。结论VEGF+936C/T基因多态性的基因型检测可能对判断NHL患者疾病侵袭性具有指导意义。

Objective To discuss the association between vascular endothelial growth factor（VEGF） gene ＋ 936C/T polymorphism and the clinical characteristics of non-Hodgkin lymphoma（NHL）. Methods Polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） was used to detect VEGF＋ 936C/T genotypes in NHL patients and unrelated healthy controls; NHL patients were divied to T-cell type and B-cell type, Ⅰ ,Ⅱ staging and Ⅲ , Ⅳ staging, bone marrow infiltration group and non-bone marrow infiltration group. The genotype distribution and allelic frequencie of the whole subgroup of NHL patients were detected. Results There was no significant difference in the frequency of VEGF ＋ 936C/T genotype between NHL patients and healthy controls（ P 〉0.05）. ＋ 936TT genetype was lower in patients than in B-cell patients（2.0% vs 7.1%, P〈0.05） ;the TT genotypes of ＋936C/T were more incidence in patients with B cell. ＋936TT genetype was lower in Ⅲ, Ⅳ staging than in Ⅰ, Ⅱ staging（3.4% vs 8.3%, P〈0.05） ; ＋936 T allele was lower in Ⅲ, Ⅳ staging than in Ⅰ, Ⅱ staging（17.1% vs 22.7%, P d0.05） ;holding ＋ 936TT genetype and T allele was associated with lower clinical staging in NHL patients. The frequency of TT genetype in bone marrow infiltration group was lower than in non-bone marrow infiltration group（2.2% vs 6.7%, P 〈0.05）. Patients with TT genetype may be asscociated with decrease bone marrow infiltration. Conclusion Determination of VEGF＋936C/T genetype may he used as a stratification marker to predicate high-risk individuals for NHL.