利用RNAi技术改良淀粉品质的研究进展

Research Advance of RNA Interference Technology in Starch Quality Improvement

RNA干涉(RNAi)是由dsRNA引起的序列特异性基因沉默现象,在动、植物和真菌中广泛存在并被证实。具有的特异性、稳定性、高效、快速以及不改变基因组的遗传组成等特性,为RNAi这一新技术在植物的遗传改良等方面提供了一个强有力的手段。重点从RNAi的发现与淀粉品质相关的淀粉合成酶及其利用RNAi在提高直链淀粉含量、降低直链淀粉含量等品质改良研究方面进行了回顾并对其存在的问题作了初步探讨。

RNA interference （RNAi）is a phenomenon of special post-transcriptional gene silencing mediated by induced dsRNA with endogenous or exogenous homogenous sequences,and a lot of researches have been applied to demonstrate it as a reserved mechanism for gene regulation in animals, plants and fungi. As its has characteristics such as specific, stability, efficient change genome of genetic composition,the new technology-RNAi is a effective method in plant genetic improvement. This paper introduced the discovery of RNAi,amylosynthetase and quality improvement in amylocellulose content by RNAi, and the problem about RNAi technology also was preliminarily discussed in this paper.