基于氨单加氧酶基因的自养脱氮菌群结构分析

Phylogenetic Analysis based on the amoA Gene of Ammonia Oxidizers in an Autotrophic Nitrogen-Removal Reactor

全程自养脱氮是一种在高氨氮低溶氧条件下完全由自养菌群作用脱除氮素的现象。以全程自养脱氮污泥为研究对象,特异性扩增氨单加氧酶活性基因amoA片段,建立克隆文库并对克隆序列进行系统发育学分析,考察全程自养脱氮系统从建立到退化过程中氨氧化菌的结构变迁。结果表明:Nitrosomonas oligotropha和Nitrosomonas europaea细菌是系统中的主要氨氧化菌,而随着系统的退化前者逐渐被后者完全取代,而氨氧化菌的种群变迁可能并不是全混流系统全程自养脱氮效率下降的原因。

In order to analyze ammonia-oxidizing population in deammonification-sludge, a partial stretch of the gene encoding the active-site polypeptide of ammonia monooxygenase （amoA） was amplified and the gene libraries were constructed. The result of gene sequences and phylogenetic analysis showed that two species of ammonia-oxidizing bacteria, including Nitrosomonas oligotropha and Nitrosomonas europaea are the dominant species in the deammonifieation reactor. With the degeneration, Nitrosornonas oligotropha were taken place with Nitrosornonas europaea,which also suggested that the changing of ammonia-oxidizing population may not cause the degeneration of deammonification reactor.