硫酸镁预处理对小鼠全脑缺血再灌注损伤后一氧化氮及一氧化氮合酶的影响

Effect of magnesium sulfate pretreatment on content of nitrous oxide and activity of nitric oxide synthase following global cerebral ischemic reperfusion injury in mice

目的:探讨硫酸镁(MgSO4)预处理对全脑缺血再灌注小鼠一氧化氮(NO)、一氧化氮合酶(NOS)活性的影响。方法:小鼠50只随机均分为假手术组、再灌组和硫酸镁预处理低、中、高剂量组,制作全脑缺血再灌注损伤模型。动态监测脑电图变化,观察病理形态学变化,检测脑组织NO含量和NOS、诱导型NOS(iNOS)活性。结果:硫酸镁预处理各组脑组织缺血损伤病理学改变轻于再灌组。假手术组及硫酸镁预处理各组脑组织NO含量和NOS、iNOS活性均低于缺血再灌组(P<0.05～P<0.01)。结论:硫酸镁预处理可减轻神经元损伤程度,对缺血再灌注脑损伤具有保护作用,其机制可能与降低NOS及iNOS活性、减少NO含量有关。

Objective ：To study the effect of Magnesium sulfate（ MgSO4 ） pretreatment on the content of nitrics oxide（NO） and the activitiy of nitric oxide synthase（NOS） following global cerebral ischemia repeffusion （I/R） injury in mice. Methods：The mice were randomly divided into sham operation group, I/R group and pretreatment groups （pretreated with low, medium and high doses of MgSO4 ）. Global cerebral ischemia-reperfusion injury model was made. The change of EEG was monitored and the pathological change in morphology was observed. The content of NO and the activity of NOS and inducible NOS （iNOS） were also detected, Results ： The change of ischemic impairment in MgSO4 pretreatment groups was lighter than that in I/R group. The contents of NO and the activities of NOS and iNOS in the sham operation group and MgSO4 pretreatment groups were all significantly lower than those of I/R group（ P 〈 0.05 - P 〈 0.01 ）. Conclusions ： MgSO4 pretreatment may reduce the degree of neuron injury and have protective effect on cerebral ischemia-reperfusion injury. The mechanism may be associated with the decrease of the content of NO as well as the activities of NOS and iNOS.