生物芯片法在结核分支杆菌检测中的应用价值探讨

Clinical value of Biochip detection of mycobacterium in sputum

目的探讨生物芯片法在结核分支杆菌诊断中的价值。方法对生物芯片法的特异度、重复性和灵敏度进行方法学评价,并用生物芯片法检测79例临床怀疑结核的患者标本和56例非结核患者标本,将其结果同传统抗酸染色法进行比较。结果在方法学实验中,生物芯片法重复性好,特异性高,灵敏度高。临床试验中,79例怀疑结核标本,生物芯片法检出率为49.4%(39/79)高于抗酸染色法19.0%(15/79),差异有统计学意义(χ2=13.87,P<0.05);而56例非结核患者检测结果均为阴性。结论生物芯片法是一种快速、灵敏度好、特异性高、重复性好的结核分支杆菌辅助诊断方法。同时生物芯片法能区分结核分支杆菌和非结核分支杆菌,在流行病学、临床诊断和治疗上有着重要意义。

Objective To investigate clinical value of Bioehip detection of myeobacterium. Methods The specificity, sensitivity and reproducibility of the bioehip method were evaluated. The specimens from 79 cases suspected tuberculosis patient and 56 cases non tuberculosis patients were detected by biochip and acid -fast stain. Then analyze and compare the results of the two methods. Results The biochip method had high sensitivity,good specificity, and good reproducibility. The Positive rate of Biochip 49. 4% （39/79）from suspected tuberculosis patient was higher than that of acid fast staining 19. 0% （15/79） and there was a significant difference （Х^2 =：13.81 ,P〈0.05）. Conclusion The Biochip can detect mycobaeterium rapidly with high specificity and sensitivity than acid-fast staining. It can be applied to clinical diagnosis.