不同慢性肝病患者自身抗体的表达差异分析

Analysis of the expression of auto-antibodies in patients with different chronic liver diseases

目的探讨自身免疫性肝病与慢性病毒性肝炎患者血清中自身抗体的表达差异及诊断应用价值。方法采用间接免疫荧光法和免疫印迹条带法对48例自身免疫性肝炎(AIH)、31例原发性胆汁性肝硬化(PBC)、55例慢性病毒性肝炎患者检测ANA、SMA、AMA、pANCA、AMA-M2、SLA/LP、LKM-1和LC-1等自身抗体。结果AIH组以高滴度的ANA、SMA、pANCA为主,检出率为71%、65%、66%,与慢性病毒性肝炎组比较,差异有统计学意义(P<0.05);PBC组以ANA、AMA、AMA-M2为主,检出率为45%、96.7%、93.5%,显著高于慢性病毒性肝炎组(P<0.01)。结论自身抗体的联合检测对自身免疫性肝病的诊断及鉴别诊断有重要意义。

Objective To research the characteristics and diagnostic value of auto antibodies in patients with autoimmune liver disease and chronic virus hepatitis. Methods ANA,SMA, pANCA, AMA subtype AMA-M2, anti-SLA/LP,anti-LKM-1 and anti- LC-1 in serum of 48 patients with autoimmune liver disease（AIH ）,31patients with primary biliary cirrhosiand （PBC） and 55 patients with chronic virus disease were tested by indirect immunofluorescence （IIf） and Immunoblot Assay respectively. Results The positive rate of auto-antibodies in autoimmune liver disease （AIH） was significantly higher than that in chronic virus disease. The positive rate of ANA, SMA and pANCA were 71%, 65 %, 66 % respectively. The positive rate of primary biliary cirrhosiand （PBC） was significantly higher than that in chronic virus disease. The positive rate of ANA,AMA and AMA-M 2 in PBC group were 45 % ,96.7 % and 93.5 % respectively. Conclusion It was clinically significant for diagnosis of autoimmune hepatic disease to identify the type of auto-antibodies.