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嗜热菌Lon蛋白酶的功能分析 被引量：2

Characterization of Lon Protease from Thermus thermophilus HB8
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摘要 Lon蛋白酶是一种在各种生物体内广泛分布并且具有多种生物功能的蛋白质.以嗜热栖热菌(Thermus thermophilus HB8)的Lon蛋白酶(TTLon)为研究对象,将TTLon蛋白酶的编码基因克隆至pTrc His载体,并在大肠杆菌中进行了成功表达,采用Ni-NTA亲和层析对其进行分离纯化,并通过一系列实验证明Lon蛋白酶能够与ATP结合,具有依赖ATP的蛋白酶活性和依赖ATP的分子伴侣活性. Lon proteases are conserved in all living organisms and play important biological roles.With the material of Lon protease from Thermus thermophilus HB8,the gene was cloned and connected to the plasmid pTrcHis.TTLon protease was successfully expressed in E.coli.The recombinant protein was purified with affinity chromatography（Ni-NTA）.A series of experiments showed that the TTLon can bind with ATP,the activity of protease and the activity of molecular chaperone were both ATP-dependent.
作者 王昕 刘晓晴
机构地区 首都师范大学生命科学学院
出处 《首都师范大学学报（自然科学版）》 2009年第5期35-39,53,共6页 Journal of Capital Normal University：Natural Science Edition
基金 国家自然科学基金资助(Nr.30870053)
关键词 Lon蛋白酶 ATP依赖型蛋白酶 分子伴侣. Lon protease ATP-dependent protease molecular chaperone.
分类号 Q556.3 [生物学—生物化学]
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参考文献14

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同被引文献14

  • 1SmithCK,Whnert J,SauerRT,Schwalbeb H.Assignments of the 1H,13C,and15N resonances of the substrate-binding SSD domain from Lon protease.Journa of Biomolecular NMR,2001,21:387-388.
  • 2Tsilibaris V,Maenhaut-Michel G,Van Melderen L.Biological roles of the Lon ATP-dependent protease.Research in Microbiology,2006,157:701-713.
  • 3Heuveling J,Possling A,Hengge R.A role for Lon protease in the control of the acid resistance genes of Escherichia coli.Molecular Microbiology,2008,69(2):534-547.
  • 4Rotanova1 TV,Melnikov EE,Khalatova1 AG,et al.Classification of ATP-dependent proteases Lon and comparison of the active sites of their proteolytic domains.Eur J Biochem,2004,271:4865-4871.
  • 5Van Melderen L,Thi HM,Lecchi P,et al.ATP-dependent degradation of CcdA by Lon protease.Effects of secondary structure and heterologous subunit interactions.The Journal of Biology Chemistry,1996,271(44):27730-27738.
  • 6Li M,Rasulova F,Melnikov EE,et al.Crystal structure of the N-terminal domain of E.coli.Lon protease.Protein Science,2005,14:2895-2900.
  • 7Rotanova TV,Melnikov EE,Khalatova AG,et al.Classification of ATP-dependent proteases Lon and comparison of the active sites of their proteolytic domains.Eur J Biochem,2004,271:4865-4871.
  • 8Botos I,Melnikov EE,Cherry S,et al.The Catalytic Domain of Escherichia coli Lon protease has a unique fold and a Ser-Lys dyad in the active site.The Journal of Biological Chemistry,2004,279:8140-8148.
  • 9Nomura K,Kato J,Takiguchi N,et al.Effects of inorganic polyphosphate on the proteolytic and DNA-binding activities of Lon in Escherichia coli.The Journal of Biological Chemistry,2004,279:34406-34410.
  • 10Lu B,Yadav S,Shah PG,et al.Roles for the human ATP-dependent Lon protease in mitochondrial DNA maintenance.The Journal of Biological Chemistry,2007,282:17363-17374.

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首都师范大学学报（自然科学版）
2009年 第5期

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