摘要
目的:探讨淫羊霍苷抑制胃癌细胞系AGS粘附和移动的作用及机制。方法:不同浓度淫羊霍苷作用AGS细胞24h后,粘附实验检测细胞粘附率,划痕损伤实验检测迁移速度。结果:2、4、8、12、16μg/ml淫羊霍苷处理24h后,AGS细胞粘附率分别为73.65%、46.24%、43.28%、41.59%和20.36%,总体呈下降趋势,4μg/ml组与2μg/ml和16μg/ml组比较差异有显著统计学意义(P<0.01),与8μg/ml和12μg/ml组比较差异无统计学意义(P>0.05)。4μg/ml淫羊霍苷处理24h后AGS细胞迁移速度明显降低(P<0.01)。结论:淫羊霍苷通过抑制AGS细胞粘附和运动来发挥对肿瘤转移的抑制作用。
Objecctive:To explore the inhibitive effect and mechanism of Icariin on adhesion and migration of AGS cell.Methods:Adhesive ratio was detected by adhesion assay,migration rate was measured by wound healing assay.Results:After AGS cells were treated with Icariin of 2,4,8,12,16μg/ml for 24h,adhesive ratios were 73.65%,46.24%,43.28%,41.59%,20.36% respectively.Compared with the 2μg/ml and 16μg/ml groups,the adhesive ratio of 4μg/ml group was significantly different(P<0.01),but with the 8μg/ml group and 12μg/ml group,the value of 4μg/ml group had no significant difference(P>0.05).After AGS cells were treated with 4μg/ml Icariin for 24h,the migration rate was markedly reduced(P< 0.01).Conclusion:Icariin can inhibit adhesion and migration of AGS cells so that it may play an anti-metastatic role.
