龟甲竹染色体C-分带、荧光原位杂交及其核型分析

Giemsa C-banding,FISH and Karyotype Analysis of Phyllostachys pubescens var.heterocycla Chromosome

利用G iem sa C-分带和荧光原位杂交的方法对龟甲竹根尖细胞有丝分裂中期染色体进行了研究。结果表明:龟甲竹的核型公式为2n=48=26m+14 sm+4 st(2SAT)+4 t,核型分类属ⅡB型;带型公式为2n=48=4C I+2C I+T+2CT++10CT++4 I+2 I++6T++6C+8T+2 IT+2 I+T,每条染色体都显示出了可以明显区别的特征带,带纹强弱差异明显。以45S rDNA为探针对龟甲竹根尖染色体进行了荧光原位杂交,杂交位点定位于1对染色体上。将染色体C-带带型和45S rDNA FISH带型相结合可以将龟甲竹的每条染色体区分开。

Giemsa C-banding and FISH analysis of the Ph. pubescens Far. heterocycla chromosomes from root tips were carried out in this research. The results showed that the C-banding karyotype would be 2n =48 = 4CI ＋ 2CI, T ＋ 2CT＋ ＋ 10CT ^＋ ＋ 4I ＋ 2I^＋ ＋ 6T^＋ ＋ 6C ＋ 8T ＋ 2IT ＋ 2I , T. The specific C-banding points on each chromosome could be distinguished and the differences of C-banding intensity also could be recognized. 45S rDNA was used as probe for FISH analysis, and one couple of hybridization signals was displayed on the chromosome centrimere. In this research we have a conclusion that chromosomes of Ph. pubescens Far. heterocycla can be easily distinguished and identified by Giemsa C-banding and FISH approaches.