摘要
The embryogenic callus induction and shoot regeneration were studied systematically in elite clone GL9 of Eucalyptus cultivated widely in south China.We investigated TDZ 0.02-0.05 mg·L-1 was the proper concentration.The callus induction rate was 92.9%—100%.When TDZ concentration was higher than 0.1 mg·L-1,the axillary bud germination was completely inhibited.TDZ 0.02 mg·L-1 with combinations of CoCl2 0.125 mg·L-1 could induct the embryogenic callus.The direct regeneration rate was 13.39%±1.03%,and with combinations of NAA 0.1mg·L-1 could not differentiate directly from callus,but higher regeneration rate(20.2%±13.3%) could be obtained by transferring callus onto regeneration medium.The size of callus can increase to 1.4 fold of its original size in the first subculture in modified MS+TDZ 0.02 mg·L-1 +NAA 0.1 mg·L-1medium and the average number of deep-pink-coloured masses of embryogenic cells on each callus was 8.4.In the second and third subculture,callus stopped growing further and the number of masses of embryogenic cells decreased gradually.Regeneration system could lay a good foundation for further transformation research.
The embryogenic callus induction and shoot regeneration were studied systematically in elite clone GL9 of Eucalyptus cultivated widely in south China. We investigated TDZ 0. 02 - 0. 05 mg · L^-1 was the proper concentration. The callus induction rate was 92.9%--100%. When TDZ concentration was higher than 0.1 mg · L^-1, the axillary bud germination was completely inhibited. TDZ 0.02 mg· L^-1 with combinations of COC120. 125 mg· L^-1 could induct the embryogenic callus. The direct regeneration rate was 13.39% - 1.03%, and with combinations of NAA 0.1 mg· L^-1 could not differentiate directly from callus, but higher regeneration rate (20.2% - 13.3% ) could be obtained by transferring callus onto regeneration medium. The size of callus can increase to 1.4 fold of its original size in the first subculture in modified MS + TDZ 0.02 mg · L^-1 + NAA 0.1 mg · L^-1 medium and the average number of deep-pink-coloured masses of embryogenic cells on each callus was 8.4. In the second and third subculture, callus stopped growing further and the number of masses of embryogenic cells decreased gradually. Regeneration system could lay a good foundation for further transformation research.
出处
《林业科学研究》
CSCD
北大核心
2009年第5期740-743,共4页
Forest Research
基金
国家林业局948项目"桉树转基因技术及抗病育种基因质粒引进"(2006-4-70)
科研院所基本科研费项目"桉树转基因技术及转基因育种研究"(2007-23)
关键词
TDZ
巨尾桉GL9
愈伤诱导率
再生率
TDZ
Eucalyptus grandis × E. urophylla GL9
callus initiation rate
regeneration rate