白花丹素的体外肾毒性及酸性成纤维细胞生长因子对白花丹素所致肾损伤的保护作用

In vitro nephrotoxicity of plumbagin and protective effect of acid fibroblast growth factor

目的:观察白花丹素在体外对人胚肾细胞293的毒性,以及酸性成纤维细胞生长因子(aFGF)对白花丹素所致肾损伤的保护作用。方法:采用四噻唑蓝(MTT)法检测不同浓度白花丹素对人胚肾细胞293的毒性,以及aFGF对白花丹素IC50值的影响,并测定培养上清液中超氧化物歧化酶(SOD)、丙二酰二醛(MDA)和乳酸脱氢酶(LDH)的活性。结果:白花丹素在体外对人胚肾细胞293的毒性呈现剂量依赖性,24、48和72 h的IC50值分别为(150.421±3.014)、(88.426±1.965)和(84.811±1.035)μg/mL。在aFGF作用下,24、48和72 h的IC50值分别升至(342.624±2.887)(、176.835±1.097)和(133.278±1.124)μg/mL。aFGF保护组与白花丹素损伤组的SOD、MDA和LDH测定结果有显著性差异(P<0.05)。结论:白花丹素可抑制人胚肾细胞293的增殖,对肾脏有一定的毒性。aFGF对白花丹素所致肾损伤有明显的保护作用。

Objective：To investigate the nephrotoxicity of plumbagin on human embryonic kidney cell 293 in vitro and protective effects of acid fibroblast growth factor（aFGF） on the cells.Methods：MTT method was used to determine nephrotoxicity of different concentrations of plumbagin on human embryonic kidney cell 293.The effect of aFGF on IC50 value of different concentrations of plumbagin was detected.The levels of SOD,MDA and LDH were determined by conventional methods.Results：Plumbagin had a dose-dependent toxicity on human kidney cell 293.IC50 values at 24,48 and 72 h were（150.421±3.014）,（88.426±1.965） and（84.811±1.035） μg/mL in plumbagin treatment group respectively,while those in aFGF treatment group were（342.624±2.887）,（176.835±1.097） and（133.278±1.124） μg/mL.There was obvious difference in the levels of SOD,MDA and LDH between the two groups（P〈0.05）.Conclusion：Plumbagin has certain toxicity on human kidney cell 293,while aFGF has protective effects against plumbagin nephrotoxicity.