用RP-HPLC法测定三黄片中四种组分的含量

Determination of contents of four components in Sanhuang tablets by RP-HPLC method

目的：建立RP-HPLC法同时测定三黄片中黄芩苷、小檗碱、大黄素和大黄酚的含量。方法：色谱柱为KromasilKR100-5 C18柱（250 mm×4.6 mm,5μm）;流动相：乙腈（A）∶3%乙酸水溶液（B）;梯度洗脱程序：A（%）∶B（%）=23∶77（0-9 min）,流速1 mL/min,A（%）∶B（%）=28∶72（10-21 min）,流速0.7 mL/min,A（%）∶B（%）=66∶34（22-35 min）,流速1 mL/min;检测波长270 nm;柱温35℃。结果：四组分均达到基线分离,各组分的平均回收率为97.00%-102.55%,方法的精密度、稳定性和重现性良好。结论：本方法快速、可靠、简便,具有应用价值。

Objective：To establish a RP-HPLC method for simultaneous determination of contents of four components in Sanhuang tablets.Methods：HPLC was used to detect the contents of the four components：baicalin,berberine,emodim and chrysophanol.The study was carried out on a Kromasil KR100-5 C18 column（250 mm×4.6 mm,5 μm） with acetonitrile（A）-3% acetic acid water solution（B） gradient elution system as mobile phase.A（%）∶B（%） was 23∶77 in 0-9 min with flow rate at 1 mL/min,A（%）∶B（%）=28∶72 in 10-21 min with flow rate at 0.7 mL/min and A（%）∶B（%）=66∶34 in 22-35 min with flow rate at 1 mL/min.The detective wavelength was 270 nm and the column temperature was 35 ℃.Results：The four components were separated on the baseline.The average recovery rates were in the range of 97.00%-102.55%.The accuracy,repeatability and stability of the method were good.Conclusion：This method is rapid,reliable,simple and applicable.