中国和国际豌豆核心种质群体结构与遗传多样性差异分析

Differentiation on Population Structure and Genetic Diversity of Pea Core Collections Separately Constituted from Chinese Landraces and International Genetic Resources

通过对中国和国际栽培豌豆(Pisum sativum L.)核心种质群间SSR等位变异数(NA)、有效等位变异数(NE)、有效等位变异所占比重(NE/NA)、等位基因丰度(AR)、基因多样性指数(GD)、Shannon′s信息指数(I)的比较,发现中国核心种质的遗传多样性指标均高于国际核心种质。中国和国际核心种质群在11个位点间存在等位变异种类的差异,属于两个明显不同的基因库,其遗传多样性差异达到显著水平。群体结构分析将核心种质划分成3个组群,组群1代表典型的中国核心种质,组群2与组群3代表不同类型的国际核心种质;组群1内种质间的平均遗传距离远高于组群2和组群3,表明中国核心种质基因型间亲缘关系明显远于国际核心种质间的亲缘关系。

Based on SSR markers, the observed number of alleles （NA） , the effective number of alleles （NE） , the ratio of NE/NA, the allelic richness （AR） , the gene diversity （GD） and the Shannon＇s information index （I） of pea core collections constituted from Chinese landraces and international genetic resources, were thoroughly compared. The value of above parameters on genetic diversity detected from Chinese local core collection, were all higher than the value of those parameters from international core collection. The difference between Chinese local and international core collections appeared in 11 SSR loci, and the two core collections were sorted into two gene pools with significantly differentiation in genetic diversity. The population structure analysis divided all the genotypes from core collections into three populations, population 1 referred to typical core collection of Chinese local landraces, population 2 and population 3 referred to intemational core collection of different genetic resources types. The genetic distance within population 1 was much larger than that within population 2 and population 3, and this reflected farther genetic relationship among genotypes within core collection of Chinese local landraces than that within international core collection.