摘要
目的采用高效液相色谱法测定棕榈地塞米松脂质体中棕榈地塞米松的含量。方法色谱柱为C18柱(250mm×4.60mm,5μm),流动相为色谱纯的乙腈-异丙醇(17∶3),柱温25℃,流速1.0mL/min,检测波长239nm,进样量20μL。结果棕榈地塞米松质量浓度的线性范围为10～100μg/mL(r=0.9991),平均回收率为99.85%,RSD为0.76%(n=9)。结论该方法操作简单,精密度好,结果准确可靠,适用于脂质体中棕榈地塞米松的含量测定。
Objective To determine the content of dexamethasone palmitate in liposome. Methods HPLC was used with C18 column （250mm ×4.6 mm, 5 μm） and the mobile phase consisted of acetonitrile and isopropylalcohol （17 ： 3）, the flow rate was 1.0 mL/min, the column temperature was 25 ℃, the determination wavelength was at 239 nm, and the injection volume was 20 μL. Results The method offered the good linearity within the range of 10- 100 μg/mL for dexamethasone palmitate （r=0. 999 1）. The average recovery rate was 99. 85%, RSD was 0.76% （n = 9）. Conclusion This method is simple, accurate and reliable for the determination of liposomal dexamethasone palmitate formulations.
出处
《中国药业》
CAS
2009年第19期30-31,共2页
China Pharmaceuticals
