高效液相色谱法同时检测4种硝基咪唑类药物的研究

HPLC simultaneous determination of the four nitroimidazoles formulation

目的建立同时检测4种硝基咪唑类药物甲硝唑、替硝唑、奥硝唑、塞克硝唑的方法。方法采用高效液相色谱法，色谱柱：Thermo C8（250mm×4．6mm，5μm）；流动相：0．05mol／L磷酸二氢钾（磷酸调pH3．5k乙腈：甲醇（76：5：19）为流动相，流速：1．0ml·min^-1；柱温40℃；检测波长：318nm；进样量：20μl。结果本研究建立的检测方法能在同一色谱条件下把4种硝基咪唑类药物甲硝唑、替硝唑、奥硝唑、塞克硝唑完全分离开来。结论该检测技术具有快速、简便、灵敏、重复性好、便于操作，专属性强等优点。

Objective To establish a checked-technology of HPLC for four nitroimidazoles formulation in the same position.Methods The HPLC analysis was carried on a Thermo C8（250 mm ×4.6mm,5μ m）.The mobile phase was 0.05mol.l-1KH2PO4（added H3PO4 to Ph3.5）-Acetonitrile-Methanol（75：5：19） at a flow rate of 1. 0 ml·min-1,inject 20μl sample into for analysis.The detection wavelength was 318 nm and the column tempera- ture was 40℃.Results The established technology can be used for the analysis of the four nitroimidazoles formulation （metronidazole,tinidazole,ornidazole,secnidazole）.Conclusions The technology is high specificity,accurate,simple, and fast.