乌司他丁对脓毒症大鼠肠道肠三叶因子mRNA表达的影响

The effects of ulinastatin on the expression of intestinal trefoil factor family mRNA in the rat model of sepsis

目的探讨脓毒症大鼠肠道肠三叶因子mRNA表达变化及乌司他丁对其的影响。方法60只SD大鼠随机分为对照组、脓毒症组、预处理组及治疗组（n=15）。后三组采用盲肠结扎穿孔术（cecal ligation and puncture，CLP）制作大鼠脓毒症模型。预处理组在CLP前2h经尾静脉注射UT125，000u/Kg，治疗组在CLP后2h经尾静脉注射UT150，000u／Kg。于模型建立后12h取回肠黏膜，观察其病理改变并以RT—PCR法检测TFF3mRNA的表达。结果TFF3mRNA在脓毒症组显著下降（P〈0．05），预处理组及治疗组较脓毒症组有明显升高（P〈0．05），预处理组较治疗组明显升高（P〈0．05）。结论脓毒症大鼠TFF，mRNA表达明显下降，乌司他丁可显著上调其表达，保护肠黏膜，预防用药较治疗给药可能更有意义。

Objective To investigate the change of the expression of intestinal trefoil factor family mRNA and effects of ulinastatin on it in the rat model of sepsis.Methods Sixty Sprague-Dawley rats were randomly divided into control, sepsis, pretreated and treated groups （n = 15）. Sepsis was induced in the later three groups by cecal ligation and puncture（ CLP ）. The pretreated groups were received 25 000u/Kg ulinastatin at 2 hours before operation and the ulinastatin treated groups were received 50 000u/Kg ulinastatin at 2 hours after operation.Some ileum mucosas were taken at 12h after C LP. The pathological change was observed and the expression of trefoil factor family mRNA was detected with RT-PCR. Results The expression of TFF3 mRNA in sepsis group significantly decreased compared to control one（P〈 0.05）. Pretreated and treated groups significantly increased compared to sepsis group （P〈 0.05）; Pretreated groups significantly increased compared to treated groups （P 〈 0.05）. Conclusions The expression of intestinal TFF3 mRNA significantly decreased in sepsis, which could be improved by the treatment of ulinastatin leading to significantly intestinal mucosal protection. The pretreatment may be more effective than the therapeutic treatment in the rat model of sepsis.