多通道流动电泳的pH值场模型

pH VALUE DISTRIBUTION MODEL FOR MULTICHANNEL FLOW ELECTROPHORESIS

建立了描述制备多通道流动电泳pH值分布的理论模型，并进行了计算。结果表明，模型在较大范围内（操作电流20mA～80mA，进样流量1ml·min^(-1)～5ml·min^(-1)，蛋白质浓度低于1.0mg·ml^(-1)）与实验值符合得很好。

A mathematical model for simulating the longitudinal distribution of pH in MFE（Multichannel Flow Electrophoresis) is proposed on the basis of mass transfer analysis of electrophoresis and electrolysis equilibrium of electrolytes.This model was used for selecting suitable operating conditions to establish an expected pH gradient with conventional electrolyte,which is of essential importance in achieving a high resolution in an electrophoresis process conducted in multicompartment electrolyzer.A numerical solution was developed on the basis of Gear method.The calculated results agreed well with the experimental data in a wide range of applied potential and protein concentration in solution.This model may also be applied to other ampholyte-free electrophoresis processes conducted in the multicompartment electrolyzer.