乙肝病毒X蛋白对E—cadherin和T—cadherin表达的调节作用

Hepatitis B virus X protein regulates the expression of E -cadherin and T -cadherin

目的 体外研究乙肝病毒X蛋白（HBx）对E-钙黏蛋白（E—cadherin）和T-钙黏蛋白（T—cadherin）表达的调节作用。方法建立稳定表达HBx基因的HepG2细胞系（HepG2-HBx），稳定转染空质粒pcDNA3．1的HepG2细胞系作为对照（HepG2-pcDNA3．1），分别用荧光实时定量（Real—time）PCR和Western—blot技术检测转染前后E—cadherin和T—cadherin在mRNA及蛋白水平表达变化。结果以HepG2-pcDNA3．1细胞为参照，转染HBx基因后的HepG2-HBx细胞E—cadherinmRNA和蛋白水平分别为其（42．28±8．73）％和（35．28±4．33）％，而T—cadher—in则分别为其（4．47-4-0．78）和（5．11±0．83）倍，差异有统计学意义（P〈0．05）。结论HBx下调E—cadherin的表达，上调T—cadherin的表达，其机制可能为HBx促进E—eadherin向T—cadherin转换。

Objective To study the effect of hepatitis B virus X protein （HBx） on the expression of E - cadherin and T - cadherin in vitro. Methods HepG2 - HBx cell line with stable expression of HBx gene was established, and HepG2 - pcDNA3. 1 cell line transfected with empty plasmid served as a control. The mRNA and protein levels of E - cadherin and T - cadherin expression were detected by real - time PCR and Western blot. Results As compared with control cells, the mRNA and protein levels of E - cadherin in HepG2 - HBx cells were decreased [ （ 42.28 ± 8.73 ） % and （ 35.28 ± 4.33 ）% respectively] , but the mRNA and protein levels of T- cadherin in HepG2 -HBx cells were increased [ （ 4.47 ± 0.78 ） and （ 5.11 ±0. 83 ） folds respectively ]. The differences were of statistical significance（ P 〈 0.05 ）. Conclusion HBx down - regulated the expression of E - cadherin, and up - regulated the expression of T - cadherin. HBx may play an important role in promoting the caherin switch from E - cadhefin to T - cadherin.