全反式维甲酸对脐血红系祖细胞增殖过程中HOXB6基因表达的影响

Effect of All-trans Retinoic Acid on Expression of HOXB6 Gene in the Process of Cell Multiplication of Cord BFU-E,CFU-E

探讨脐血造血干祖细胞向红系增殖过程中HOXB6 mRNA表达及全反式维甲酸对HOXB6 mRNA表达的影响.采用体外培养技术,以全反式维甲酸持续干扰造血干祖细胞,观察集落生成情况,采用实时荧光定量PCR技术检测造血祖细胞增殖分化过程中HOXB6基因的表达水平,用DNA相对拷贝数和RNA表达相对量表示HOXB6基因相对表达量.结果表明,人类造血干祖细胞向红系祖细胞增殖分化过程中,各祖细胞HOXB6基因均表达,与正常对照组比较,全反式维甲酸可上调HOXB6基因的表达,说明HOXB6可能是造血干祖细胞向红系祖细胞正常增殖分化过程中的调控基因之一.

To observe the expression level of HOXB6 gene mRNA on the differentiation and proliferation of hematopoietie stem-progenitor cell （HSPC） to colony forming erythroid progenitor （CFU-E）, and whether the proliferation progress was affected by all-trans retinoie acid （ATRA）. In vitro technology was used to obsen,e the elonogenieity when ATRA, continuously disturbed hematopoietie stem cell; besides this, real-t quantitative PCR was used to measure the expression level during the proliferation and differentiation of hemopoietie progenitor eell. Relative eopy number of DNA and relative RNA expression were used to show relative expression quantities of HOXB6. The results are the following： firstly, during the differentiation and proliferation of hematopoietie stem-progenitor cell to colony forming unit-T lymphocyte or erythroid progenitor in vitro, the expression of HOXB6 is significantly positive; secondly, compared with the expression of HOXB6 of normal group, the expression of HOXB6 of group ATRA is remarkably up-regulated （ P 〈 0. 05）. The findings show that Homeobox genes （HOX） do have a regulatory function in the differentiation preeess of hematopoiesis when hematopoietie stem cell nonnally proliferates ,and differenliales into BFU-E, and CFU-E ATRA can up-reguiate the expression of HOXB6.