大豆球蛋白经口诱发五指山小型猪过敏反应的实验研究

Anaphylactic reactions in WZS minipig orally induced by glycinin

目的探讨大豆球蛋白(11S)诱导五指山小型猪食物过敏反应的合适剂量和适宜观察时点。方法12头45日龄五指山小型猪随机分为对照组、4%11S和8%11S大豆蛋白致敏组,每组4头,通过胃肠道致敏(0～10天)和反复经口激发(16～18天、31天)诱导致敏反应,从临床症状、皮刺反应、血清IgG、IgE含量、组胺含量、肥大细胞数目等指标进行观察和实验研究。结果接受11S大豆蛋白经口致敏的仔猪出现不同程度的腹泻症状,皮肤试敏试验呈不同程度的阳性反应;第19天和32天4%11S致敏组血清中IgG、IgE抗体和组胺含量均高于对照组,第19天含量达到高峰;4%11S致敏组空肠粘膜下层肥大细胞数目低于对照组,空肠组胺含量与空肠粘膜层和粘膜下层肥大细胞数均呈负相关,(P<0.05或P<0.01)。结论由11S经口诱发的五指山小型猪食物致敏模型属IgE介导的Ⅰ型过敏反应类型,4%剂量较8%剂量过敏反应更明显,激发期是观察和比较仔猪过敏反应的较佳时点,过敏反应可能与小肠肥大细胞活化有关。

Objective To explore the suitable dosage and observation period of anaphylactic reactions in WZS minipigs induced by glycinin（ 11S）. Methods Twelve 45-day-old WZS minipigs were randomly divided into three groups （control group, 4% 11S group and 8% 11S group, n = g）, which were respectively orally sensitized（0 - 10d） and orally challenged（16- 18d, 31d） with diets containing 0%, 4% 11S or 8% 11S soybean proteins to induce anaphylactic reactions. Clinical symptoms, skin prick reactions, serum IgG, serum IgE, histamine, mast cell in the small intestine were observed or measured. Results Diarrhea and positive skin prick reactions at different degrees were observed in 11S groups. Compared with the control group, serum IgG, IgE and histamine levels of 4% llS group at 19d and 32d were significantly higher（ P 〈 0.05）, and reached the peak when llS groups were orally challenged at 19d. The jejunum histamine level was negatively correlated with the mast cell numbers in the jejunum mucosa and sub-mucosa（ P 〈 0.05）. Compared with the control, the mast cell number in the jejunum sub-mucosa of 4% 11S group was decreased （ P 〈 0.05）. Conclusion The WZS minipig allergic reaction orally induced by glycinin could be type I hypersensitivity mediated by IgE, reaction induced by 4 % dosage glycinin could be more strong than that of 8 % dosage, and stimulating phase could be a suitable observation period for anaphylactic reactions which could be related with the activation of mast cells in the small intestine.