丙烯酰胺致L-02肝胎细胞损伤作用的研究

Study on DNA damage in L-02 cell induced by acrylamide

目的观察丙烯酰胺(AA)对L-02肝胎细胞株细胞周期、细胞凋亡的影响以及对DNA的损伤作用。方法以不同终浓度AA(0.01、0.1、0.5、1.0、2.5、5.0和7.5mmol/L)对L-02细胞进行染毒,12h和24h后分别检测细胞存活率、细胞周期、细胞凋亡和DNA损伤。结果与对照组相比,低剂量(0.01mmol/L和0.1mmol/L)染毒细胞存活率略有升高,中剂量(0.5、1.0和2.5mmol/L)、高剂量(5.0mmol/L和7.5mmol/L)则显著降低(P<0.05);G1期细胞含量随染毒剂量的升高逐渐降低,中、高剂量组(>1.0mmol/)G1期细胞含量显著低于对照组(P<0.05),S期细胞含量则相反;中、高剂量(>2.5mmol/L)染毒组细胞凋亡率显著高于对照组(P<0.05);中、高剂量(>1.0mmol/L)染毒细胞拖尾DNA含量以及OTM值显著升高(P<0.05)。结论AA可引起细胞周期紊乱及细胞凋亡,并且对DNA有较强的损伤作用。

Objective To investigate the cell cycle, cell apoptosis and DNA damage induced by acrylamide （AA）in L-02 human liver embryo cell line. Methods The rate of cell survivors, cell cycle, cell apoptosis and DNA damage were measured respectively after L-02 cell were exposed to 0.01, 0.1, 0.5, 1.0, 2.5, 5.0 and 7.5mmol/L AA for 12h and 24h in vitro. Results The rates of cellular survivors in the low （0.01mmol/L and 0.1mmol/） dose AA-treated group were higher than those in control group, but those in the middle （0.5, 1.0 and 2.Smmol/L） and high （5.0mmol/L and 7.5mmol/L） dose AA-treated groups were significantly lower than those in control group （P 〈 0.05）. The rate of cell in G1 stage was reduced along with the promotion of AA, and the middle and high （ 〉 1.0 mmol/L） dose AA-treated groups were significantly lower than the control group （ P 〈 0.05 ）, while the rate of cell in S stage was in reverse. The percentages of apoptosis were significantly higher than that of the control group in the high dose AA-treated groups （ P 〈 0.05 ） . The percents of tail DNA and Oliver Tail Moment （OTM） were significantly higher than that of the control group in the middle and high （ 〉 1.0 mmol/L） dose AA-treated groups （P 〈 0.05）. Conclusion AA could induce the confusion and apoptosis of L-02 cell, and has impairment effect on DNA.