植物激素的反相高效液相色谱法分离和测定

Separation and Determination of Eight Plant Hormones by Reversed Phase High Performance Liquid Chromatography

探讨了分离和测定８种植物生长激素的反相高效液相色谱法。结果表明：以含有０．８％醋酸的甲醇和水作为流动相，采用梯度洗脱，可在１６ｍｉｎ内将８种物质完全分离并可进行定量测定，线性关系良好。方法具有快速、简便、准确的特点。

In this paper, reversed phase high performance liquid chromatographic technique was used for the separation and determination of eight plant hormones. Methanol water acetic acid system was chosen as the mobile phase. The effects of different separation conditions, such as the methanol and acetic acid concentrations in mobile phase, on the retention behaviours of eight plant hormones in this system were studied. The general trends in retention behaviours could be correlated to the methanol concentration in mobile phase. The experimental results showed that the optimum separation was achieved with following gradient elution condition: 0 3 minutes, 70%(water percentage in mobile phase), 3 13 minutes, 70% 20%, 13 48minutse, 20%. Benzene was added to be as the internal standard. Under this experimental condition, the eight plant hormones could be separated completely and detected quantitatively at 260nm within 16 minutes. The calibration curves for the eitht compounds gave linearity over a wide range. The correlation coefficients of each components were r(ZT)=0.997 1, r(GAs)=0.999 9, r(K)=0.999 7, r(BA)= 0.999 5 , r(IAA)=0.999 8, r(IPA) =0.998 2, r(IBA)= 0.999 5 and r(NAA)=0.999 5. The method is rapid, simple and efficient. It is a suitable method for the accurate determination of gibberellic acid (GA) and alpha naphthaleneacetic acid (α NAA) in products for agricultural use.