毛细管电色谱中缓冲液离子强度对ODS柱分离行为的影响

Influence of Ion Strength on the Separation Behavior on ODS Column in Capillary Electrochromatography

介绍了毛细管电色谱中流动相的选择以及缓冲液的不同离子强度对ＯＤＳ柱分离行为的影响。

Capillary electrochromatography is the combination of high performance liquid chromatography and capillary electrophoresis. In capillary electrochromatography, the choice of mobile phases depends on the one hand, the stationary phases and samples to be separated, and on the other hand, the conducting buffer. In this paper, the mixture of organic solvent and buffer was used as mobile phases, and the different ion strength of buffer was found to influence the separation. How the ion strength influences the separation behavior on a packed ODS column is discussed.