几种化合物对东北主要土壤芳基硫酸酯酶活性影响的比较

Response of arylsulfatase in main soils of northeast China to several regulators

本着探索化合物添加对土壤芳基硫酸酯酶活性的作用效果的目的,采用室内模拟培养试验方法,以东北黑土、白浆土、棕壤和褐土为供试对象,以还原型谷胱甘肽、ATP和辅酶I 3种化合物作为酶活性调节剂,研究了激活剂对4种土壤芳基硫酸酯酶活性的调节,为进一步研究土壤芳基硫酸酯酶活性的激活调节提供一定参考。结果表明,棕壤在3 d后表现了良好的效果,直达近3个月的时间,棕壤添加ATP和还原型谷胱甘肽的效果好于辅酶I。棕壤添加还原型谷胱甘肽后第3、5、7、15、30、60、90 d芳基硫酸酯酶活性分别为对照的1.09、1.21、1.54、1.28、1.22、0.95、0.96倍,有效硫含量则分别为对照的1.16、1.12、.1.15、1.11、1.08、1.07、1.06倍。棕壤添加ATP第3、5、7、15、30、60、90 d后芳基硫酸酯酶活性分别为对照的1.23、1.49、1.43、1.41、1.30、1.09、1.03倍,但有效硫含量与对照相当。研究所选化合物对供试土壤没有同样的激活效果,需要进一步大量筛选以找到相应的调节剂再对其机理进行深入研究。在施入土壤的肥料中添加有机化合物以激活土壤芳基硫酸酯酶活性,对加速土壤有机硫的矿化,为植物补充硫素营养具有重大意义,对农业生产也有重要的参考价值。

Sulfur is necessary nutrition element for plant growth, while soil lack sulfur badly and soil sulfur turn into restricted factor for crop yields and quality inch by inch. Soil arylsulfatase may be one biology index reflecting soil quality, which can catalyze mineralization of soil organic sulfur and play an important role in sulfur biochemistry circulation and plants＇ sulfur nutrition. It should be benefit for agricultural production evidently on the assumption that inorganic compounds were added into fertilizers activating soil arylsulfatase activity to accelerate mineralization of soil inorganic sulfur. Aimed to exploring response of soil arylsulfatase to several regulators, the regulation of reductive glutamate, coenzymes - I and ATP on soil arylsulfatase of black soil, albic soil, brown soil and cinnamon soil were studied under a simulated condition. Arylsulfatase and available sulfur in brown soil was enhanced.