大肠埃希菌耐药性及ESBLs、AmpC酶检测临床分析

Detection on the drug resistance of Escherichia coli and analysis the incidence of ESBLs and AMPC enzyme

目的研究笔者所在医院2008年1月至2008年12月临床分离的大肠埃希菌耐药性及产超广谱β内酰胺酶(ESBLs)和质粒(AmpC)酶的发生率。方法收集笔者所在医院2008年1月至2008年12月大肠埃希菌53株,用K-B法测定细菌对头孢他啶等17种抗菌药物的敏感性,采用头孢西丁改良三维试验检测ESBLs和AmpC酶。结果 53株大肠埃希菌对美罗培南、亚胺培南、哌拉西林/他唑巴坦、头孢哌酮/舒巴坦、阿米卡星、头孢美唑、头孢西丁、头孢他啶、头孢吡肟的耐药率在0%～18.9%,对其他抗生素耐药率在30.2%～71.7%。53株大肠埃希菌中,24株ESBLs阳性,检出率为45.3%;4株AmpC酶阳性,检出率为7.5%。结论笔者所在医院ESBLs发生率较高,产ESBLs是大肠埃希菌对多种抗生素耐药的主要原因。

Objective To study on the drug resistance of clinically isolated Escherichia coil and analysis the incidence of ESBLs,AMPC enzyme production in our hospital during January,2008 - December,2008. Methods 53 escherichiacoli were collected in in our hospital during January, 2008 - December,2008. The bacterial sensitivity to 17 kinds of antibiotics including ceftazidime was determined by K - B method and the ESBLs ,AMPC enzymes were determined improved eefoxitin three - dimensional test. Results The drug resistance of 53 Eseherichia eoli is about 0 - 18.9% to Meropenem, imipenem, piperacillin / tazobaetam, cefoperazone / sulbactam, amikaein, cefmetazole, cefoxitin, ceftazidime, cefepime. In contrast, the bacterial drug resistance is about 30.2% -71.7% to other antibiotics. In 53 escheriehia coli eases, 24 ESBLs is positive with 45.3% detection rate and the positive rate of 4 AMPC is 7.5%. Conclusion The ESBLs production of escherichia coli is high in our hospital, which is the main reason of multiple antibiotics resistance.