虫草胶囊菌粉的鉴定与分析

Identification and Analysis of Cordyceps Products

目的探讨建立一种虫草胶囊菌粉真伪的检测方法。方法以95%乙醇、乙酸乙酯和Tris-HCl对胶囊菌粉产品处理后再提取DNA,排除虫草胶囊菌粉中杂质对PCR扩增的干扰,采用PCR扩增该菌粉ITS1-5.8S-ITS2区的序列。结果鉴定分析了3个不同厂家虫草胶囊产品的发酵菌粉,系统发育学分析表明样品1胶囊生产菌为中国被毛孢(Hirsutella sinensis),属于国家卫生部允许的可用于保健食品的真菌菌株;样品2和样品3分别为西藏虫草(Cordyceps gracilis)和蛹虫草(Cordyceps militaris),均与产品名称"冬虫夏草"(Cordyceps sinensis)不符,且不属于国家卫生部允许的保健食品菌株。结论该方法简单易行,可以有效的鉴定市售虫草相关产品的真伪,为该类产品的真伪鉴定开拓了新的思路。

Objective To develop a method for true-false distinguish of Cordyceps capsule products.Method Before extracting the genomic DNA,three samples were pretreated by 95% alcohol,ethylacetate and Tris-HCl for removing impurities in the capsules,and then the ITS1-5.8S-ITS2 region sequence was amplified from the genomic DNA.Results Three Cordyceps capsule products sold in the market were identified by phylogenetic analysis. The phylogenetic analysis results showed that sample 1 belonged to Hirsutella sinensis, which was permitted for Cordyceps capsule manufacture by the Ministry of Health, P. R. China. while ＂sample 2＂ and ＂sample 3＂ were Cordyceps gracilis and Cordyceps militaris. Conclusion The method could be simple, credible. It is a new idea for true-false distinguish of Cordyceps capsule products.