广西地区人群乙肝疫苗无、弱应答与HLA-DRB1*11等位基因的相关性研究

Association of non-or hypo-response to hepatitis B vaccination with HLA-KRB*111 allele in the population of Guangxi province

目的研究广西地区人群接种重组乙肝疫苗后无、弱应答与HLA-DRB1*11等位基因的相关性。方法选取广西籍912名健康大学生,按0,1,6方案标准全程接种重组乙肝疫苗,接种半年后采血检测血清中抗-HBs水平。对首次检测抗-HBs<10 mIU/ml者再行接种重组乙肝疫苗20 ug。4周后抗-HBs仍小于10 mIU/ml的65人作为无、弱应答组,随机抽取首次检测抗-HBs>10 mIU/ml的中、强应答者96名作为对照组。应用PCR-SSP方法检测外周血HLA-DRB1*11等位基因。结果HLA-DRB1*11等位基因在无、弱应答组的携带率为16.92%,在中、强应答组的携带率为14.58%,两组比较无统计学意义(P>0.05)。结论HLA-DRB1*11等位基因频率与广西地区人群乙肝疫苗免疫无、弱应答无明显相关性。

Objective To study the association between non-or hypo-response to hepatitis B vaccination and HLA-DRB1＊11 allele in the population of Guangxi Province. Methods 912 healthy undergraduate students were subjected to a standard course of immunization with recombinant hepatitis B vaccination at the month of 0,1, and 6. Half a year after the last immunization, the subjects were examined their serum level of anti- HBs antibody. The ones whose anti-HBs was less than 10mIU/ml at the first time test were selected to receive another 20ug doses of recombinant hepatitis B vaccination and a detection of serum HBV markers again four weeks later. 65 persons whose anti-HBs antibody still remained less than 10mIU/ml were considered as non-or hypo-responders group ,and 96 subjects of the medium or hyper-responders （anti-HBs 〉 10mIU/ml at the first detection） were drawn randomly from the same cohort as the controls. HLA-DRB1＊11 allele was detected by polymerase chain reaction using sequence specific primer （PCR-SSP）. Results The allelie frequencies of HLA-DBB1 ＊ 11 in the non-or hypo-responders group and the controls were 16.92% and 14.58% respectively. There was no significant difference between the two groups （16.92% vs 14.58% ,P 〉0.05 ）. Conclusion HLA-DRB1＊11 is not associated with the non-or hypo-response to hepatitis B vaccination in the population of Guangxi Province.