HPLC法比较非酒精性脂肪性肝病患者和正常人血浆中维生素E含量

Compare the contents of vitamin E in healthy human plasma with the nonalcoholic fatty liver disease patients by HPLC

目的验证非酒精性脂肪性肝病与血浆中维生素E含量的关系。方法依利特RP18色谱柱(3.9 mm×250 mm,5μm),柱温:30℃,流动相:甲醇-水(体积比为95∶5),流速:1.2 mL.min-1,检测波长:在0～7 min采用325 nm波长、在7.01～12.00 min采用280 nm波长进行双波长检测,视黄醇醋酸酯为内标对比15例正常人和15例非酒精性脂肪性肝病病人中的VE浓度。结果维生素E保留时间为9.28 min,浓度线性为1～16 mg.L-1,Y=4.896 3x+0.226 4,R2=0.998 2。15例健康成人血浆维生素E平均值为(7.61±1.61)mg.L-1;15例肝硬化病人血浆维生素E平均值为(0.486±0.071)mg.L-1。结论非酒精性脂肪性肝病病人血浆维生素E含量明显低于正常人。

Objective The experimental verifies the relationship between the nonalcoholic fatty liver disease （NAFLD） patients and their vitamin E contents in plasma. Methods The assay was conducted on the elite C18（250 mm ×3.9 mm,5 μm）columnn with methanol-water（95： 5）as the mobile phase. The flow rate is 1.2 mL. min^- 1, and the column temperature is 30℃. The retinyl acetate was taken as the internal standard. The detection wavelength was set at 325 nm for retinyl acetate and 280 run for vitamin E. The experimental compares the content of vitamin E of the 15 cases of healthy human and 15 cases of patients. Results The retention time for vitamin E is 9. 28 min. The linearity range is 1 - 16 mg .L^-1 with a regressive equation of Y =4. 896 3 x ＋0. 226 4 ,R^2 =0. 998 2. Fifteen cases of healthy adults plasma vitamin E average is（7.61 ± 1.61 ）mg. L^-1 ;15 healthy adult plasma vitamin E average is （0. 486±0. 071 ）mg. L^-1. Conclusions The vitamin E plasma of NAFLD patients content is lower than normal.