凹顶藻萜类化合物对小鼠H_(22)肿瘤生长及VEGF、PCNA表达的影响

Effects of Laurencia Terpenoids on Tumor Growth and Expression of VEGF and PCNA in H_(22) Mice

目的:观察凹顶藻提取物(Laurencia terpenoid extract,LET)对接种H22细胞小鼠的肿瘤生长及血管内皮细胞生长因子(VEGF)、增殖细胞核抗原(PCNA)表达的影响。方法:昆明小鼠随机分为5组(10只/组),即模型组、LET低中高剂量组(25、50、100mg/kg·d-1)、环磷酰胺组(CTX对照组)。各组小鼠左前腋下皮下接种H22肝癌细胞,第二天除模型组外,其余4组分别以不同剂量的LET、CTX灌胃,于15天后处死,完整剥离出肿瘤,称重,计算抑瘤率。免疫组化法测定肿瘤组织中VEGF、PCNA的表达。结果:LET低、中、高剂量组小鼠H22肿瘤质量增长均较模型组缓慢(P<0.05),抑瘤率分别为27.5%、34.9%、41.4%;同时LET中、高剂量组VEGF阳性表达较模型组显著减少(P<0.05),低剂量组未见明显变化,但LET各剂量组PCNA阳性表达较模型组显著减少(P<0.05)。结论:LET各剂量组可以抑制小鼠H22肿瘤的生长,具有较高的抑瘤活性,且中、高剂量组能抑制VEGF的表达(P<0.05),低、中、高剂量组能抑制PCNA的表达(P<0.05)。LET对肿瘤组织VEGF、PCNA表达的抑制作用可能是其抗H22肿瘤及抗血管生成的一个重要原因。

Objective： To observe the effect of Laurencia terpenoid extract（LET）on tumor growth, VEGF and PCNA in H22 mice. Method： KM mice were randomly divided into five groups： the model group, the LET group （25、50、100mg/kg·d^-1）, the cyclophos-phamide group （the CTX control group） and each group had ten mice. H22 Cells were inoculated subcutaneously into left anteromedial of KM mice. Animals of four groups （except the model group） were treated with ig LET of different dosage and CTX on the second day and executed after 15 days. We weighed the tumor which was stripped completely and calculated the tumor inhibition rate. Simultaneously the expression of VEGF and PCNA in tumor tissue were determined by immunohistochemistry. Result： In vivo, the increase of H22 tumor weight in LET groups was slower than that in model group （P〈0.05）, and the tumor inhibition rate was 27.5%, 34.9%, 41.4% respectively. The treatment with mid and high-dose LET inhibited the expression of VEGF （P〈0.05）, while there is no significant changes in low-dose group. Different dose LET inhibited the expression of PCNA （P〈0.05） within tumor. Conclusion： LET inhibits tumor growth possessing higher tumor inhibition rate, as well as PCNA expression in H22 mice （P〈0.05）. Mid and high-dose LET inhibited the expression of VEGF（P〈0.05）. The depressant effect may be an important reason for opposing H22 tumor and angiogenesis.