摘要
目的:检测鼠抗人G-csf单克隆抗体的相对亲和力。方法:采用间接酶联免疫吸附试验(ELISA)。结果:各株单抗结合抗原的相对亲和力不同,根据50%最大结合浓度分为2组,其中4株杂交瘤上清McAbs与纯化腹水McAbs测得结果基本吻合。结论:实验结果为合理运用这些单抗提供了重要依据。
Objective:To measure the relative affinity of anti G csf monoclonal antibodies.Method:Indirect enzyme linked immunosorbent assay (ELISA) was used.Results:The affinity of various McAbs to the same antigen was different and some of the difference was very evident.Six McAbs were divided into two groups by analyzing their 50% maximum binding.The relative affinity of McAbs in the culture supernate was consistent with that in the purified ascites.Conclusion:The study provides useful information for the rational application of these McAbs.
出处
《滨州医学院学报》
1998年第6期524-525,共2页
Journal of Binzhou Medical University